Angebote zu "Spectrometry" (19 Treffer)

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Using Mass Spectrometry for Biochemical Studies...
98,77 € *
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This thesis reports studies on the substrate specificity of crucial ketosynthase (KS) domains from trans-AT Polyketide Synthases (PKSs). Using a combination of electrospray ionisation-mass spectrometry (ESI-MS) and simple N-acetyl cysteamine (SNAC) substrate mimics, the specificity of a range of KS domains from the bacillaene and psymberin PKSs have been succsessfully studied with regard to the initial acylation step of KS-catalysis.In addition, the ability to alter the substrate tolerance of KS domains by simple point mutations in the active site has been demonstrated. A series of acyl-ACPs have been synthesised using a novel methodology and employed to probe the substrate specificity of both KS domains and the previously uncharcterised acyl hydrolase domain, PedC.KS-catalysed chain elongation reactions have also been conducted and monitored by ESI-MS/MS. All KS domains studied exhibited higher substrate specificity at the elongation step than in the preceeding acylation step. Furthermore, a mechanism of reversible acylation is proposed using the PsyA ACP1-KS1 di-domain. The findings in this thesis provide important insights into mechanisms of KS specificity and show that mutagenesis can be used to expand the repertoire of acceptable substrates for future PKS engineering.

Anbieter: Dodax AT
Stand: 28.01.2020
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Atomic Physics with Heavy Ions
123,90 € *
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This book is devoted to one of the most active domains of atomic physic- atomic physics of heavy positive ions. During the last 30 years, this terrain has attracted enormous attention from both experimentalists and theoreti cians. On the one hand, this interest is stimulated by rapid progress in the development of laboratory ion sources, storage rings, ion traps and methods for ion cooling. In many laboratories, a considerable number of complex and accurate experiments have been initiated, challenging new frontiers. Highly charged ions are used for investigations related to fundamental research and to more applied fields such as controlled nuclear fusion driven by heavy ions and its diagnostics, ion-surface interaction, physics of hollow atoms, x-ray lasers, x-ray spectroscopy, spectrometry of ions in storage rings and ion traps, biology, and medical therapy. On the other hand, the new technologies have stimulated elaborate theo retical investigations, especially in developing QED theory, relativistic many body techniques, plasma-kinetic modeling based on the Coulomb interactions of highly charged ions with photons and various atomic particles - electrons, atoms, molecules and ions. The idea of assembling this book matured while the editors were writ ing another book, X-Ray Radiation of Highly Charged Ions by H. F. Beyer, H. -J. Kluge and V. P. Shevelko (Springer, Berlin, Heidelberg 1997) covering a broad range of x-ray and other radiative phenomena central to atomic physics with heavy ions.

Anbieter: Dodax AT
Stand: 28.01.2020
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Atomic Physics with Heavy Ions
124,04 € *
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This book is devoted to one of the most active domains of atomic physic- atomic physics of heavy positive ions. During the last 30 years, this terrain has attracted enormous attention from both experimentalists and theoreti cians. On the one hand, this interest is stimulated by rapid progress in the development of laboratory ion sources, storage rings, ion traps and methods for ion cooling. In many laboratories, a considerable number of complex and accurate experiments have been initiated, challenging new frontiers. Highly charged ions are used for investigations related to fundamental research and to more applied fields such as controlled nuclear fusion driven by heavy ions and its diagnostics, ion-surface interaction, physics of hollow atoms, x-ray lasers, x-ray spectroscopy, spectrometry of ions in storage rings and ion traps, biology, and medical therapy. On the other hand, the new technologies have stimulated elaborate theo retical investigations, especially in developing QED theory, relativistic many body techniques, plasma-kinetic modeling based on the Coulomb interactions of highly charged ions with photons and various atomic particles - electrons, atoms, molecules and ions. The idea of assembling this book matured while the editors were writ ing another book, X-Ray Radiation of Highly Charged Ions by H. F. Beyer, H. -J. Kluge and V. P. Shevelko (Springer, Berlin, Heidelberg 1997) covering a broad range of x-ray and other radiative phenomena central to atomic physics with heavy ions.

Anbieter: Dodax
Stand: 28.01.2020
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Mass Spectrometry of Proteins and Peptides
124,59 € *
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When the last edition of this book was published in 2000, the field of proteomics was in its infancy. At that time, multidimensional liquid chromatographic separations were being introduced as an alternative to traditional gel-based techniques for separating complex protein and peptide mixtures prior to mass spectrometric detection. Today, this approach – referred to as shotgun proteomics – is considered routine for lar- scale global analyses of protein mixtures. Now in its adolescence, proteomics is fundamentally transforming biological and medical research. Much of this transformation can be attributed to technological advancements, particularly in mass spectrometry. Much wider accessibility of hi- resolution and mass measurement accuracy instrumentation in recent years has ini- ated a new revolution in the field by providing more reliable data and shifting the focus from cataloging proteins to precisely quantifying changes in protein abundance over time and in response to stimuli. Advanced mass spectrometers and novel ion d- sociation schemes such as electron transfer/capture dissociation make it possible to venture boldly into the maze of protein posttranslational modifications, which are an integral component of understanding functional proteomics in the spatial and t- poral domains. Another area that has benefited from these advancements is top-down proteomics, an emerging method essential for characterizing various protein variants that has potentially high impact in biomedical research.

Anbieter: Dodax
Stand: 28.01.2020
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Mass Spectrometry of Proteins and Peptides
192,59 € *
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When the last edition of this book was published in 2000, the field of proteomics was in its infancy. At that time, multidimensional liquid chromatographic separations were being introduced as an alternative to traditional gel-based techniques for separating complex protein and peptide mixtures prior to mass spectrometric detection. Today, this approach - referred to as shotgun proteomics - is considered routine for lar- scale global analyses of protein mixtures. Now in its adolescence, proteomics is fundamentally transforming biological and medical research. Much of this transformation can be attributed to technological advancements, particularly in mass spectrometry. Much wider accessibility of hi- resolution and mass measurement accuracy instrumentation in recent years has ini- ated a new revolution in the field by providing more reliable data and shifting the focus from cataloging proteins to precisely quantifying changes in protein abundance over time and in response to stimuli. Advanced mass spectrometers and novel ion d- sociation schemes such as electron transfer/capture dissociation make it possible to venture boldly into the maze of protein posttranslational modifications, which are an integral component of understanding functional proteomics in the spatial and t- poral domains. Another area that has benefited from these advancements is top-down proteomics, an emerging method essential for characterizing various protein variants that has potentially high impact in biomedical research.

Anbieter: Dodax
Stand: 28.01.2020
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Mass Spectrometry of Proteins and Peptides
124,60 € *
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When the last edition of this book was published in 2000, the field of proteomics was in its infancy. At that time, multidimensional liquid chromatographic separations were being introduced as an alternative to traditional gel-based techniques for separating complex protein and peptide mixtures prior to mass spectrometric detection. Today, this approach – referred to as shotgun proteomics – is considered routine for lar- scale global analyses of protein mixtures. Now in its adolescence, proteomics is fundamentally transforming biological and medical research. Much of this transformation can be attributed to technological advancements, particularly in mass spectrometry. Much wider accessibility of hi- resolution and mass measurement accuracy instrumentation in recent years has ini- ated a new revolution in the field by providing more reliable data and shifting the focus from cataloging proteins to precisely quantifying changes in protein abundance over time and in response to stimuli. Advanced mass spectrometers and novel ion d- sociation schemes such as electron transfer/capture dissociation make it possible to venture boldly into the maze of protein posttranslational modifications, which are an integral component of understanding functional proteomics in the spatial and t- poral domains. Another area that has benefited from these advancements is top-down proteomics, an emerging method essential for characterizing various protein variants that has potentially high impact in biomedical research.

Anbieter: Dodax AT
Stand: 28.01.2020
Zum Angebot
Mass Spectrometry of Proteins and Peptides
197,99 € *
ggf. zzgl. Versand

When the last edition of this book was published in 2000, the field of proteomics was in its infancy. At that time, multidimensional liquid chromatographic separations were being introduced as an alternative to traditional gel-based techniques for separating complex protein and peptide mixtures prior to mass spectrometric detection. Today, this approach - referred to as shotgun proteomics - is considered routine for lar- scale global analyses of protein mixtures. Now in its adolescence, proteomics is fundamentally transforming biological and medical research. Much of this transformation can be attributed to technological advancements, particularly in mass spectrometry. Much wider accessibility of hi- resolution and mass measurement accuracy instrumentation in recent years has ini- ated a new revolution in the field by providing more reliable data and shifting the focus from cataloging proteins to precisely quantifying changes in protein abundance over time and in response to stimuli. Advanced mass spectrometers and novel ion d- sociation schemes such as electron transfer/capture dissociation make it possible to venture boldly into the maze of protein posttranslational modifications, which are an integral component of understanding functional proteomics in the spatial and t- poral domains. Another area that has benefited from these advancements is top-down proteomics, an emerging method essential for characterizing various protein variants that has potentially high impact in biomedical research.

Anbieter: Dodax AT
Stand: 28.01.2020
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Using Mass Spectrometry for Biochemical Studies...
106,99 € *
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Using Mass Spectrometry for Biochemical Studies on Enzymatic Domains from Polyketide Synthases ab 106.99 € als pdf eBook: . Aus dem Bereich: eBooks, Fachthemen & Wissenschaft, Wissenschaften allgemein,

Anbieter: hugendubel
Stand: 28.01.2020
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Using Mass Spectrometry for Biochemical Studies...
117,49 € *
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Using Mass Spectrometry for Biochemical Studies on Enzymatic Domains from Polyketide Synthases ab 117.49 € als gebundene Ausgabe: 1st ed. 2016. Aus dem Bereich: Bücher, Wissenschaft, Biologie,

Anbieter: hugendubel
Stand: 28.01.2020
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