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The Effect of Mutating the PDZ domains within s...
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PDZ domains are one of the most common protein-protein interaction domains in human. PDZ domain containing 2 protein (PDZD2) contains 6 PDZ domains and is highly expressed in pancreatic beta cells. It undergoes cleavage in the endoplasmic reticulum to produce a secreted form PDZD2 (sPDZD2) which retains the last two PDZ domains of PDZD2. sPDZD2 is a candidate beta cell regulatory factor. In current study, two mutated forms of sPDZD2 were generated with alterations in the amino acid sequence within the carboxylate-binding loop of one of the two PDZ domains, either at the PDZ5 or at the PDZ6 domain. Current study revealed that both the PDZ5-mutated and PDZ6-mutated sPDZD2 proteins failed to exert the insulinotropic effect that the wildtype sPDZD2 had on INS-1E cells when added exogenously in the culture medium. Both mutant proteins also failed to rescue the silencing action of siRNA on the insulinotropic effect of endogenous PDZD2 in INS-1E cells. These results suggest that intact carboxylate-binding loops of both PDZ5 and PDZ6 domain are crucial for the insulinotropic effect of sPDZD2 exerting on INS-1E cells.

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Stand: 28.01.2020
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Wat, Z: Effect of Mutating the PDZ domains with...
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Erscheinungsdatum: 08/2011, Medium: Taschenbuch, Einband: Kartoniert / Broschiert, Titel: The Effect of Mutating the PDZ domains within secreted PDZD2, Titelzusatz: on its insulinotropic action in INS-IE cells, Autor: Wat, Zee man, Winnie, Verlag: LAP Lambert Acad. Publ., Sprache: Englisch, Rubrik: Genetik // Gentechnologie, Seiten: 96, Informationen: Paperback, Gewicht: 159 gr, Verkäufer: averdo

Anbieter: averdo
Stand: 28.01.2020
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The Effect of Mutating the PDZ domains within s...
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The Effect of Mutating the PDZ domains within secreted PDZD2 ab 49 EURO on its insulinotropic action in INS-IE cells

Anbieter: ebook.de
Stand: 28.01.2020
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The Effect of Mutating the PDZ domains within s...
43,99 € *
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PDZ domains are one of the most common protein-protein interaction domains in human. PDZ domain containing 2 protein (PDZD2) contains 6 PDZ domains and is highly expressed in pancreatic beta cells. It undergoes cleavage in the endoplasmic reticulum to produce a secreted form PDZD2 (sPDZD2) which retains the last two PDZ domains of PDZD2. sPDZD2 is a candidate beta cell regulatory factor. In current study, two mutated forms of sPDZD2 were generated with alterations in the amino acid sequence within the carboxylate-binding loop of one of the two PDZ domains, either at the PDZ5 or at the PDZ6 domain. Current study revealed that both the PDZ5-mutated and PDZ6-mutated sPDZD2 proteins failed to exert the insulinotropic effect that the wildtype sPDZD2 had on INS-1E cells when added exogenously in the culture medium. Both mutant proteins also failed to rescue the silencing action of siRNA on the insulinotropic effect of endogenous PDZD2 in INS-1E cells. These results suggest that intact carboxylate-binding loops of both PDZ5 and PDZ6 domain are crucial for the insulinotropic effect of sPDZD2 exerting on INS-1E cells.

Anbieter: Thalia AT
Stand: 28.01.2020
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