98,77 € *

ggf. zzgl. Versand

ggf. zzgl. Versand

This thesis reports studies on the substrate specificity of crucial ketosynthase (KS) domains from trans-AT Polyketide Synthases (PKSs). Using a combination of electrospray ionisation-mass spectrometry (ESI-MS) and simple N-acetyl cysteamine (SNAC) substrate mimics, the specificity of a range of KS domains from the bacillaene and psymberin PKSs have been succsessfully studied with regard to the initial acylation step of KS-catalysis.In addition, the ability to alter the substrate tolerance of KS domains by simple point mutations in the active site has been demonstrated. A series of acyl-ACPs have been synthesised using a novel methodology and employed to probe the substrate specificity of both KS domains and the previously uncharcterised acyl hydrolase domain, PedC.KS-catalysed chain elongation reactions have also been conducted and monitored by ESI-MS/MS. All KS domains studied exhibited higher substrate specificity at the elongation step than in the preceeding acylation step. Furthermore, a mechanism of reversible acylation is proposed using the PsyA ACP1-KS1 di-domain. The findings in this thesis provide important insights into mechanisms of KS specificity and show that mutagenesis can be used to expand the repertoire of acceptable substrates for future PKS engineering.

Anbieter: Dodax AT

Stand: 28.01.2020 Zum Angebot

Stand: 28.01.2020 Zum Angebot

100,98 € *

ggf. zzgl. Versand

ggf. zzgl. Versand

This book presents and discusses the construction of mathematical models that describe phenomena of flow and transport in porous media as encountered in civil and environmental engineering, petroleum and agricultural engineering, as well as chemical and geothermal engineering. The phenomena of transport of extensive quantities, like mass of fluid phases, mass of chemical species dissolved in fluid phases, momentum and energy of the solid matrix and of fluid phases occupying the void space of porous medium domains are encountered in all these disciplines. The book, which can also serve as a text for courses on modeling in these disciplines, starts from first principles and focuses on the construction of well-posed mathematical models that describe all these transport phenomena.

Anbieter: Dodax

Stand: 28.01.2020 Zum Angebot

Stand: 28.01.2020 Zum Angebot

100,98 € *

ggf. zzgl. Versand

ggf. zzgl. Versand

Anbieter: Dodax AT

Stand: 28.01.2020 Zum Angebot

Stand: 28.01.2020 Zum Angebot

35,00 € *

ggf. zzgl. Versand

ggf. zzgl. Versand

High Quality Content by WIKIPEDIA articles! Reduced mass is the "effective" inertial mass appearing in the two-body problem of Newtonian mechanics. This is a quantity with the unit of mass, which allows the two-body problem to be solved as if it were a one-body problem. Note however that the mass determining the gravitational force is not reduced. In the computation one mass can be replaced by the reduced mass, if this is compensated by replacing the other mass by the sum of both masses. The reduced mass is typically used as a relationship between two system elements in parallel, such as resistors, whether these be in the electrical, thermal, hydraulic, or mechanical domains. This relationship is determined by the physical properties of the elements as well as the continuity equation linking them. The two-body problem can be re-formulated as two independent one-body problems, a trivial one and one that involves solving for the motion of one particle in an external potential. Since many one-body problems can be solved exactly, the corresponding two-body problem can also be solved.

Anbieter: Dodax AT

Stand: 28.01.2020 Zum Angebot

Stand: 28.01.2020 Zum Angebot

55,90 € *

ggf. zzgl. Versand

ggf. zzgl. Versand

The research progress in the field of concentrated solar power (CSP) with photovoltaic thermal (PVT) technologies has a great impact for fulfilling today's energy demand. So this book talks about the performance of PVT system and effect of fins on the performance of the system. The numerical modelling is used to solve fluid and structural domains, and both analyses are synchronised with the help of Fluid-Structure Interface. This phenomenon is helpful to examine the effect of concentrated solar fluxes, temperature distribution and mass flow rate. The obtained results show that, there is potential enhancement in performance of PVT system using extended surfaces at 0.83 kg/sec mass flow rate. This CSP - PVT system is able to enhance and produce thermal and electrical power simultaneously.

Anbieter: Dodax

Stand: 28.01.2020 Zum Angebot

Stand: 28.01.2020 Zum Angebot

81,30 € *

ggf. zzgl. Versand

ggf. zzgl. Versand

Practical fluid flows are often associated withcomplex physical domains. Simulating such fluid flowsaccurately and efficiently remains a challenge in theComputational Fluid Dynamics. In doing so, thecomputational mesh must be able to properly representthe physical domain and be organized in an efficientway. This book describes the author s research on thedevelopment of mesh generation and multi-blockalgorithm for 2D numerical models in geometricallycomplex domains. Two improved RL elliptic generationsystems were developed by using the contributionfactors to achieve the mesh density control and theglobal balance of mesh orthogonality and smoothness.A 2D structured mesh generator--the CCHE-MESH hasbeen developed (free athttp://www.ncche.olemiss.edu/software/downloads). Amulti-block algorithm based on a consistentinterpolation method along with a Lagrangianinterpolation method was also developed. It iscapable of maintaining both mass balance and momentumbalance at the block interfaces. The proposed meshgeneration techniques and multi-block algorithm weresuccessfully applied to complex domains and producedsatisfactory results.

Anbieter: Dodax AT

Stand: 28.01.2020 Zum Angebot

Stand: 28.01.2020 Zum Angebot

34,00 € *

ggf. zzgl. Versand

ggf. zzgl. Versand

Anbieter: Dodax

Stand: 28.01.2020 Zum Angebot

Stand: 28.01.2020 Zum Angebot

57,50 € *

ggf. zzgl. Versand

ggf. zzgl. Versand

Anbieter: Dodax AT

Stand: 28.01.2020 Zum Angebot

Stand: 28.01.2020 Zum Angebot

79,00 € *

ggf. zzgl. Versand

ggf. zzgl. Versand

Anbieter: Dodax

Stand: 28.01.2020 Zum Angebot

Stand: 28.01.2020 Zum Angebot