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Using Mass Spectrometry for Biochemical Studies...
103,72 € *
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This thesis reports studies on the substrate specificity of crucial ketosynthase (KS) domains from trans-AT Polyketide Synthases (PKSs). Using a combination of electrospray ionisation-mass spectrometry (ESI-MS) and simple N-acetyl cysteamine (SNAC) substrate mimics, the specificity of a range of KS domains from the bacillaene and psymberin PKSs have been succsessfully studied with regard to the initial acylation step of KS-catalysis.In addition, the ability to alter the substrate tolerance of KS domains by simple point mutations in the active site has been demonstrated. A series of acyl-ACPs have been synthesised using a novel methodology and employed to probe the substrate specificity of both KS domains and the previously uncharcterised acyl hydrolase domain, PedC.KS-catalysed chain elongation reactions have also been conducted and monitored by ESI-MS/MS. All KS domains studied exhibited higher substrate specificity at the elongation step than in the preceeding acylation step. Furthermore, a mechanism of reversible acylation is proposed using the PsyA ACP1-KS1 di-domain. The findings in this thesis provide important insights into mechanisms of KS specificity and show that mutagenesis can be used to expand the repertoire of acceptable substrates for future PKS engineering.

Anbieter: Dodax
Stand: 24.01.2020
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Using Mass Spectrometry for Biochemical Studies...
98,77 € *
ggf. zzgl. Versand

This thesis reports studies on the substrate specificity of crucial ketosynthase (KS) domains from trans-AT Polyketide Synthases (PKSs). Using a combination of electrospray ionisation-mass spectrometry (ESI-MS) and simple N-acetyl cysteamine (SNAC) substrate mimics, the specificity of a range of KS domains from the bacillaene and psymberin PKSs have been succsessfully studied with regard to the initial acylation step of KS-catalysis.In addition, the ability to alter the substrate tolerance of KS domains by simple point mutations in the active site has been demonstrated. A series of acyl-ACPs have been synthesised using a novel methodology and employed to probe the substrate specificity of both KS domains and the previously uncharcterised acyl hydrolase domain, PedC.KS-catalysed chain elongation reactions have also been conducted and monitored by ESI-MS/MS. All KS domains studied exhibited higher substrate specificity at the elongation step than in the preceeding acylation step. Furthermore, a mechanism of reversible acylation is proposed using the PsyA ACP1-KS1 di-domain. The findings in this thesis provide important insights into mechanisms of KS specificity and show that mutagenesis can be used to expand the repertoire of acceptable substrates for future PKS engineering.

Anbieter: Dodax AT
Stand: 24.01.2020
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The Nurse Professoriate Viewed From the Lenses ...
81,30 € *
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This study focused on: how cultural domains are incarnated in the nurse professoriate's understanding and praxis of instruction, research and community service, identify the factors in the internal and external environments which influence the values of the nurse professoriate, and describe the culture of the nurse professoriate in the colleges of nursing. The narrative interpretive method of research and documentary analysis were utilized. Twenty-six nurse professoriate from both government and private colleges of nursing participated in the study with the aid of a semi-structured interview. The narrative interpretive analysis was used for analyzing the data that was divided into descriptive and interpretive phases. Data revealed the nurse professoriate's different conceptions of what constitutes teaching. Five cultural values: honesty, commitment, competence, compassion/caring were identified by the nurse professoriate. The culture of instruction, research and community service interacts with the four cultural domains which consist of energizing, edifying, preserving and connecting elements.

Anbieter: Dodax AT
Stand: 24.01.2020
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The Nurse Professoriate Viewed From the Lenses ...
79,00 € *
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This study focused on: how cultural domains are incarnated in the nurse professoriate's understanding and praxis of instruction, research and community service, identify the factors in the internal and external environments which influence the values of the nurse professoriate, and describe the culture of the nurse professoriate in the colleges of nursing. The narrative interpretive method of research and documentary analysis were utilized. Twenty-six nurse professoriate from both government and private colleges of nursing participated in the study with the aid of a semi-structured interview. The narrative interpretive analysis was used for analyzing the data that was divided into descriptive and interpretive phases. Data revealed the nurse professoriate's different conceptions of what constitutes teaching. Five cultural values: honesty, commitment, competence, compassion/caring were identified by the nurse professoriate. The culture of instruction, research and community service interacts with the four cultural domains which consist of energizing, edifying, preserving and connecting elements.

Anbieter: Dodax
Stand: 24.01.2020
Zum Angebot
Feminist Critical Discourse Analysis
57,90 € *
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The first collection to bring together well-known scholars writing from feminist perspectives within Critical Discourse Analysis. The theoretical structure of CDA is illustrated with empirical research from a range of locations (from Europe to Asia, the USA to Australasia) and domains (from parliament to the classroom, the media to the workplace).

Anbieter: Dodax
Stand: 24.01.2020
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Integrating Face and Voice in Person Perception
200,97 € *
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This book follows a successful symposium organized in June 2009 at the Human Brain Mapping conference. The topic is at the crossroads of two domains of increasing importance and appeal in the neuroimaging/neuroscience community: multi-modal integration, and social neuroscience.Most of our social interactions involve combining information from both the face and voice of other persons: speech information, but also crucial nonverbal information on the person's identity and affective state. The cerebral bases of the multimodal integration of speech have been intensively investigated, by contrast only few studies have focused on nonverbal aspects of face-voice integration.This work highlights recent advances in investigations of the behavioral and cerebral bases of face-voice multimodal integration in the context of person perception, focusing on the integration of affective and identity information.Several research domains are brought together. Behavioral and neuroimaging work in normal adult humans included are presented alongside evidence from other domains to provide complementary perspectives: studies in human children for a developmental perspective, studies in non-human primates for an evolutionary perspective, and studies in human clinical populations for a clinical perspective.Several research domains are brought together. Behavioral and neuroimaging work in normal adult humans included are presented alongside evidence from other domains to provide complementary perspectives: studies in human children for a developmental perspective, studies in non-human primates for an evolutionary perspective, and studies in human clinical populations for a clinical perspective.Several research domains are brought together. Behavioral and neuroimaging work in normal adult humans included are presented alongside evidence from other domains to provide complementary perspectives: studies in human children for a developmental perspective, studies in non-human primates for an evolutionary perspective, and studies in human clinical populations for a clinical perspective.Several research domains are brought together. Behavioral and neuroimaging work in normal adult humans included are presented alongside evidence from other domains to provide complementary perspectives: studies in human children for a developmental perspective, studies in non-human primates for an evolutionary perspective, and studies in human clinical populations for a clinical perspective.

Anbieter: Dodax
Stand: 24.01.2020
Zum Angebot
Feminist Critical Discourse Analysis
58,33 € *
ggf. zzgl. Versand

The first collection to bring together well-known scholars writing from feminist perspectives within Critical Discourse Analysis. The theoretical structure of CDA is illustrated with empirical research from a range of locations (from Europe to Asia, the USA to Australasia) and domains (from parliament to the classroom, the media to the workplace).

Anbieter: Dodax AT
Stand: 24.01.2020
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Lp-Theory of Cylindrical Boundary Value Problems
82,34 € *
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Tobias Nau addresses initial boundary value problems in cylindrical space domains with the aid of modern techniques from functional analysis and operator theory. In particular, the author uses concepts from Fourier analysis of functions with values in Banach spaces and the operator-valued functional calculus of sectorial operators. He applies abstract results to concrete problems in cylindrical space domains such as the heat equation subject to numerous boundary conditions and equations arising from fluid dynamics.

Anbieter: Dodax
Stand: 24.01.2020
Zum Angebot
Integrating Face and Voice in Person Perception
200,97 € *
ggf. zzgl. Versand

This book follows a successful symposium organized in June 2009 at the Human Brain Mapping conference. The topic is at the crossroads of two domains of increasing importance and appeal in the neuroimaging/neuroscience community: multi-modal integration, and social neuroscience.Most of our social interactions involve combining information from both the face and voice of other persons: speech information, but also crucial nonverbal information on the person's identity and affective state. The cerebral bases of the multimodal integration of speech have been intensively investigated, by contrast only few studies have focused on nonverbal aspects of face-voice integration.This work highlights recent advances in investigations of the behavioral and cerebral bases of face-voice multimodal integration in the context of person perception, focusing on the integration of affective and identity information.Several research domains are brought together. Behavioral and neuroimaging work in normal adult humans included are presented alongside evidence from other domains to provide complementary perspectives: studies in human children for a developmental perspective, studies in non-human primates for an evolutionary perspective, and studies in human clinical populations for a clinical perspective.Several research domains are brought together. Behavioral and neuroimaging work in normal adult humans included are presented alongside evidence from other domains to provide complementary perspectives: studies in human children for a developmental perspective, studies in non-human primates for an evolutionary perspective, and studies in human clinical populations for a clinical perspective.Several research domains are brought together. Behavioral and neuroimaging work in normal adult humans included are presented alongside evidence from other domains to provide complementary perspectives: studies in human children for a developmental perspective, studies in non-human primates for an evolutionary perspective, and studies in human clinical populations for a clinical perspective.Several research domains are brought together. Behavioral and neuroimaging work in normal adult humans included are presented alongside evidence from other domains to provide complementary perspectives: studies in human children for a developmental perspective, studies in non-human primates for an evolutionary perspective, and studies in human clinical populations for a clinical perspective.

Anbieter: Dodax AT
Stand: 24.01.2020
Zum Angebot